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Assistant Professor of Chemistry; Associate Scientist, James Graham Brown Cancer Center
Research Interests http://www.louisville.edu/a-s/chemistry/faculty/jga/arnezlab.htm
EducationEducation/Training
B.S., City College of New York, New York, Chemistry, 1985
Ph.D., Yale University, New Haven, CT, Molecular Biophysics & Biochemistry, 1991
Postdoc, Universite Louis Pasteur, Strasbourg, France, Structural Biology, 1992 - 1998
Research and Professional Experience
1984-1985
Research aide, Department of Chemistry, City College of New York, NY
1986-1992
Predoctoral researcher, Department of Molecular Biophysics and Biochemistry, Yale, University, New Haven, CT
1992-1998
Postdoctoral researcher, Laboratoire de Biolgie Structurale, Institut de Genetique et de Biologie Moleculaire et Cellulaire (CNRS), Strasbourg, France
1998-2002
Assistant Professor of Biochemistry, Albert Einstein College of Medicine, Bronx, NY
2002-2003
Research Assistant Professor of Biochemistry, University of Vermont, Burlington, VT
2003-Present
Assistant Professor of Chemistry, University of Louisville, Louisville, KY
Selected Awards and Profressional Honors
1985 Ward Medal in Chemistry and J. Birnbaum prize, City College of New York
1985-1988 NIH Training Grant in Molecular Biophysics and Biochemistry
1993 Association Pour la Recherche contre le Cancer Fellowship
1994-1995 Eli Lilly Fellowship
1996 Fondation pour la Cecherche Medicale Fellowship
1997-1998 Charge d’Etudes, Centre National de la Recherche Scientifique (France)
PublicationsVioque A, Arnez JG, Altman S. Protein-RNA interactions in the RNase P holoenzyme from Escherichia coli. J Mol Biol 202:835-48 1988
Baer MF, Arnez JG, Guerrier-Takada C, Vioque A, Altman S. Preparation and characterization of RNase P from Escheria coli. Meth Enzymol 181:569-82 1990
Arnez JG, Steitz TA. Crystal structure of unmodified tRNAGIn complexed with glutaminyl-tRNA synthetase and ATP suggests a possible role for pseudo-uridines in stabilization of RNA structure. Biochemistry 33:7560-67 1994
Arnex JG, Harris DC, Mitschler A, Rees B, Francklyn CS, Moras D. Crystal structure of histidyl-tRNA synthetase from E. coli complexed with histidyl-adenylate. EMBO J 14:4143-55 1995
Arnez JG, Steitz TA Crystal structures of three misacylating mutants of E. coli glutaminyl-tRNA synthetase complexed with tRNAGIn and ATP. Biochemistry 35:14725-733 1996
Arnez JG, Augustine JG, Moras D, Francklyn CS. The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase. Proc Natl Acad Sci USA 94,7144-49 1997
Arnez JG, Moras D. Structural and functional considerations of the aminoacylation reaction. Trends Biochem Sci 22, 211-16 1997
Arnez JG, Cavarelli J. Structures of RNA-binding proteins. Quart Rev Biophys 30, 195-240 1997
Arnez JG, Flanagan K, Moras D, Simonson T. Engineering a Mg2+ sie to replace a structurally conserved arginine in the catalytic center of histidyl-tRNA synthetase by computer experiments. Proteins: Struct Funct Gen 32:362-80, 1998
Arnez JG, Dock-Bregeon AC, Moras D. Glycl-tRNA synthetase uses a negatively charged pit for specific recognition and activation of glycine. J Mol Biol 286:1449-59 1999
Arnez JG. Application of the molecular replacement method to a heavy atom problem. J Appl Cryst 32:472-74, 1999
Freist W, Verhey JF, Ruhlmann A, Bauss DH, Arnez JG. Histidyl-tRNA synthetase. Biol Chem 380:623-46, 1999
Arnez JG, Sankaranarayanan R, Dock-Bregeon AC, Francklyn CS, Moras D. Aminoacylation at the atomic level in class IIa aminoacyl-tRNA sytenetases. J Biomolec Struct Dyn Conversation 11(1):23-28 2000
Shah FS, Curr KA, Hambrugh ME, Parniak M, Mitsuya H, Arnez JG, Prasad VR. Differential influence of nucleoside analog-resistance mutations K65R and L74V on the overall mutation rate and error specificity of human immunodeficiency virus type 1 RT. Biol Chem 275:27037-044 2000
Ng JD, Sauter C, Lorber B, Kirkland N, Arnez JG, Giege R. Comparative analysis of space-grown and earth-grown crystals of an aminoacyl-tRNA synthetase: space-grown crystals are more useful for structural determination. Acta Crystallogr D Biol Crystallogr 58:645-52 2002
Francklyn CS, Arnez JG. “Histidyl-tRNA Synthetase,” in The Aminoacyl-tRNA Synthetases. (Ibba M, Cusack S, Francklyn C, eds.) Landes Biosciences Publ. Georgetown, TX 2004
Contact InfoChemistry Building
Phone: 502-852-2733
Fax: (502) 852-8149
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