Hong Ye, PhD

Assistant Professor of Medicine; Associate Scientist, James Graham Brown Cancer Center

Research Program

Structural Biology

Research Interests

Structural and functional studies of signal transduction; X-ray crystallography.
The research in my laboratory is focused at understanding the structural and molecular mechanism of Notch signaling regulation primarily with X-ray crystallography combined with a variety of biophysical and biochemical methods. We are interested in the activation and regulatory processes involving different proteins, and we use thermodynamics as well as structural methods to define the interactions.

Education

B.S., Xiamen University, Xiamen, China, Cell Biology, 1987-1991
Ph.D., Keele University, Keele, UK, Biophysics, 1995-1998

Postdoctoral Fellow, Weill Medical College, Cornell University, New York, Biochemistry, 1998-2003

Research and Professional Experience
1991-1995
Assistant Researcher, Institute of Biophysics, Chinese Academy of Science, China

1996-1998
Teaching Assistant, Department of Physics, Keele University, UK

1998-2003
Postdoctoral Associate, Department of Biochemistry, Weill Medical College Cornell University

Selected Awards and Professional Honors
1995-1998
Departmental Research Studentship

1995-1998
Overseas Research Students Awards

2000-2002
Charles H. Revson Foundation fellowship

2002-2003
Keck Program Postdoctoral Fellow

Publications

Shotton MW, Pope LH, Forsyth VT, Denny R, Langan P, Ye H, Boote C.  New developments in instruments forX-ray and neutron diffraction experiments.  J Appl Crystallography 31:758, 1998

Ye H, Park YC, Kreishman M, Kieff E, Wu H.  The structural basis for the recognition of diverse receptor
sequences by TRAF2.  Molecular Cell 4:321-30, 1999

Wu H, Park YC, Ye H, Tong L.  Structure studies of human TRAF2. Cold Spring Harbor: Symposia on
Quantitative Biology.  Signaling and Gene Expression in the Immune System, 64:541-9, 1999

Park YC, Ye H, Hsia C, Segal D, Rich RL, Liou HC, Myszka DG, Wu H.  A novel mechanism of TRAF signaling
revealed by structural and functional analyses of the TRADD-TRAF2 interaction.  Cell 101:777-87, 2000

Ye H, Wu H.  Thermodynamic characterization of the interaction between TRAF2 and receptor peptides by
isothermal titration calorimetry.  Proc Natl Acad Sci USA 97:8961-6, 2000

Ye H, Arron JR, Lamothe B, Cirilli M, Kobayashi T, Shevde NK, Segal D, Dzivenu OK, Vologodskaia M, Yim M,
Du K, Singh S, Pike JW, Darnay BG, Choi Y, Wu H.  Distinct molecular mechanism for initiating TRAF6 signaling.  Nature 418:443-7, 2002

Ye H, Cande C, Stephanou N, Gurbuxani S, Larochette N, Daugas E, Garrido C, Kroemer G, Wu H.  DNA
binding as a structural requirement for apoptogenic action of AIF.  Nature Struct Biol 9(9):680-4, 2002

Ye H, Cirilli M, Wu H.  The use of genetic methods and diffraction data analysis in the crystallization of the
TRAF domain of human TRAF6.  Acta Cryst D 58:1886-8, 2002

Chung J, Park YC, Ye H, Wu H.  All TRAFs are not created equal: common and distinct molecular
mechanisms of TRAF-mediated signal transduction.  J Cell Sci 115(4):679-88, 2002

Chung J, Park YC, Ye H, Wu H. TNF receptor associated factors.  In: Cytokine Signaling Handbook, 2003

Johnson JD, Han Z, Otani K, Ye H, Zhang Y, Wu H, Horikawa Y, Misler S, Bell GI, Polonsky KS.  RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets.  J Biol Chem 279(23):24794-802, 2004.

Ye H, Chen TC, Xu X, Pennycooke M, Wu H, Steegborn C.  Crystal structure of the putative adapter protein MTH1859.  J Struct Biol 148(2):251-6, 2004.

Ye H, Maurizio C, Wu H.  The use of genetic methods and diffraction data analysis in the crystallization of the TRAF domain of human TRAF6. Acta Cryst. in press.

Contact Info

CTR Building
505 South Hancock Street
Louisville, KY 40202
Phone: (502) 852-4047

Fax: (502) 852-4311

 

 

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