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Research ProgramStructural Biology
Research Interests
(Chemistry; BCC, Pharmacology & Toxicology; Director Metabolomics): metabolomics-NMR, MS
My research interests lie in metabolomics of environmental stresses in plants, invertebrates, and mammals and how such information facilitates a mechanistic understanding of organismal response to the environment. Multinuclear and 2-dimensional NMR along with various mass spectrometric techniques are the main tools for these investigations. Recently we have been using this technology to analyze the metabolism of different kinds of cancers compared with untransformed cells, We are using isotopomer analysis to determine the relative fluxes through glycolysis, the pentose phosphate pathways and the TCA.
My research interests are in the analysis of macromolecular interactions involved in regulation of transport protein function with emphasis on transport ATPases using detergent solubilization and analytical ultracentrifugation along with other biophysical and biochemical approaches.
EducationEducation/Training
B.S., National Taiwan University, Public Health, 1973-1977
M.S., University of Hawaii, Manoa, Food Science, 1977-1978
Ph.D., University of California, Davis, Biochemistry, 1978-1983
Post-Doc, Stanford University, 1984-1986
Visiting Post-Doc, University of California, Davis, 1986
Research and Professional Experience
1986-1989
Postgraduate Research Biochemist,
University of California, Davis
1989-1998
Assistant Research Biochemist,
University of California, Davis
1998-
Associate Research Biochemist,
University of California, Davis
2002-
Associate Professor, Department of Chemistry,
University of Louisville
2002-
Scientist, James Graham Brown Cancer Center,
University of Louisville
2003-
Associate Professor, Department of Pharmacology & Toxicology,
University of Louisville
PublicationsFan TW-M, Higashi RM,Lane AN. Chemical characterization of a chelator-treated soil humate by solution-state multinuclear two-dimensional NMR with FTIR and pyrolysis-GCMS. Environ Sci Technol 34:1636-46, 2000.
Fan TW-M, Lane AN, Shenker M, Bartley JP, Crowley D, Higashi RM. Comprehensive chemical profiling of gramineous plant root exudates using high-resolution NMR and MS. Phytochem. 57:209-21, 2001.
Shenker M,Fan TWM,Crowley DE. Phytosiderophores influence on cadmium mobilization and uptake by wheat and narley plants. J Environ Quality 30:2091-8, 2001.
Larsen EH, Hansen M, Fan T, Vahl M. Speciation of selenoamino acids, selenonium ions and inorganic selenium by ion exchange HPLC with mass spectrometric detection and its application to yeast and algae. J Anal Atom Spectrom 16,:1403-08, 2001.
Fan TW-M, Teh S, Hinton DE, Higashi RM. Selenium biotransformations into proteinaceous forms by foodweb organisms of selenium-laden drainage waters in California. Aquatic Toxicology, special Se issue with Fan as guest editor, 57:65-84, 2002.
Fan TWM, Pruszkowski E, Shuttleworth S. Speciation of selenoproteins in Se-contaminated wildlife by gel electrophoresis and laser ablation-ICP-MS. J Anal Atom Spectrom. 17:1621-3, 2002.
Fan TWM, Lane AN, Ramos A, Hollingworth D. NMR determination of the secondary structure of selenocysteine insertion sequences. Biophys. J 82:637, 2002.
Fan TW-M, Lane AN, Higashi RM. In vivo and in vitro metabolomic analysis of anaerobic rice coleoptiles revealed unexpected pathways. Russ J Plant Physiol 50:787-93, 2003.
Ramos A, Lane AN, Hollingworth D, Fan TW-M. NMR determination of the secondary structure of the selenocysteine insertion element. Nucleic Acids Res 32:1746-55, 2004
Fan TW-M, Higashi RM, Lane AN. A rapid electrophoretic profiling method for thiol-rich peptides and proteins. Phytochem Anal 15(3):175-83, 2004.
Fan TW-M, Lane AN, Chekmenev E, Wittebort RH, Higashi RM. Determination of the composition and physical properties of peptides in soil organic matter. J Peptide Res 63(3):253-64, 2004.
Fan TW, Lane AN, Higashi RM. The promise of metabolomics in cancer molecular therapeutics. Curr Opin Mol Ther 6(6):584-92, 2004. Review.
Teh SJ, Deng X, Deng DF, Teh FC, Hung SS, Fan TW, Liu J, Higashi RM. Chronic effects of dietary selenium on juvenile Sacramento splittail (Pogonichthys macrolepidotus). Environ Sci Technol 38(22):6085-93, 2004.
Phone: Coming soon.
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