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Research ProgramStructural Biology
Research Interests
(Pharmacology & Toxicology; Director - Biomolecular Mass Spectrometry Facility, Chemistry.): Bone structure, MS and proteomics
My laboratory group develops and applies techniques in mass spectrometry to analysis of synthetic and naturally occurring compounds. Techniques include electron and chemical ionization, electrospray and MALDI, coupled with quadrupole, magnetic sector, ion-trap, time of flight and hybrid (MS-MS) mass analysis. These techniques are used to perform qualitative and quantitative analyses of synthetic compounds, to study expression proteomics, to define functional proteomic complexes, to study structural modifications of proteins, carbohydrates and nucleic acids and to map cell surface proteins.
EducationEducation/Training
B.A., University of Louisville, KY, Chemistry, 1977
Ph.D., University of Louisville, KY, Pharmacology, 1981
Post-Doc, Stanford University, CA, Molecular Pharmacology, 1981-1982
Research and Professional Experience
1982-1985
Research Associate in Pharmacology & Toxicology,
University of Louisville School of Medicine
1987-1991
Assistant Professor of Pharmacology & Toxicology,
University of Louisville School of Medicine
1987-1991
Assistant Professor Ophthalmology & Visual Sciences,
University of Louisville School of Medicine
1991-1997
Associate Professor of Pharmacology & Toxicology,
University of Louisville School of Medicine
1991-1997
Associate Professor Ophthalmology & Visual Sciences,
University of Louisville School of Medicine
1997-present
Professor of Pharmacology & Toxicology,
University of Louisville School of Medicine
1997-2002
Professor of Ophthalmology & Visual Sciences,
University of Louisville School of Medicine
1997-present
Director, Biomolecular Mass Spectrometry Laboratory,
University of Louisville School of Medicine
2001-present
Professor of Chemistry,
University of Louisville College of Arts and Sciences
Publications
Feltzer R, Gray RD, Pierce WM, Dean WL. Alkaline Proteinase Inhibitor of Pseudomonas aeruginosa: Interaction of Native and N-terminally Truncated Inhibitor Proteins with Pseudomonas Metalloproteinases. J Biol Chem 275(28):21002-21009, July 14, 2000
Li J, Yen TY, Allende ML, Joshi RK, Cai J, Pierce WM Jr., Jaskiewicz E, Darling DS, Macher BA, Young WW Jr. Disulfide bonds of GM2 synthase homodimers: Antiparallel orientation of the catalytic domains. J Biol Chem 275(52):41476-86, 2000
Thakkar RR, Wang OL, Zrouga M, Stillwell W, Haq A, Kissling R, Pierce WM Jr., Smith NB, Miller FN, Ehringer WD. Docosahexaenoic acid reverses cyclosporin A-induced changes in membrane structure and function. Biochim Biophys Acta1474(2):183-95, 2000.
Nerland DE, Cai J, Pierce WM Jr., Benz FW. Covalent binding of acrylonitrile to specific rat liver glutathione-S-transferases in vivo. Chem. Res. Toxicol 14:799-806, 2001
Fitzpatrick JL, Ripp SL, Smith NB, Pierce WM Jr., Prough RA. Metabolism of DHEA by Cytochromes P450 in Rodent and Human Liver Microsomal Fractions. Arch Biochem Biophys 389(2):278-87, 2001
Ping P, Zhang J, Pierce WM Jr., Bolli R. Functional Proteomic Analysis of PKCe Signaling Complexes Associated with Cardioprotection. Circ Res. 88(1):59-62, 2001
Madhavi J, Rane PY, Coxon DW, Powell RW, Klein JB, Ping P, Pierce W, McLeish KR. p38 kinase-dependent MAPKAPK-2 activation functions as PDK2 for AKT in human neutrophils. J Biol Chem 276:3517-3523, 2001
Thongboonkerd V, Luengpailan J, Cao J, Pierce WM Jr., Cai J, Klein JB, Doyle RJ. Fluoride exposure attenuates expression of Streptococcus pyogenes virulence factors. J Biol Chem 277:16599-16605, 2002
Feng W, Cai J, Pierce WM Jr., Song ZH. Expression of functional CB2 cannabinoid receptor in Pichia pastoris for purification and mass spectrometric characterization. Protein Expr Purif 26(3):496-505, 2002
Ping P, Song C, Zhang J, Guo Y, Cao X, Li RC, Wu W, Vondriska TM, Pass JM, Tang XL, Pierce WM Jr., Bolli R. Formation of protein kinase C(epsilon)-Lck signaling module confers cardioprotection. J Clin Invest 109:499-507, 2002
Singh S, Powell DW, Rane MJ, Millard TH, Trent JO, Pierce WM, Klein JB, Machesky LM, McLeish KR. Identification of the p16-Arc subunit of the Arp2/3 complex as a substrate of MAPK-activated protein kinase-2 by proteomic analysis. J Biol Chem 278(38):36410-7, 2003
Klein JB, Gozal D, Pierce WM, Thongboonkerd V, Scherzer JA, Sachleben LR, Guo SZ, Cai J, Gozal E. Proteomic identification of a novel protein regulated in CA1 and CA3 hippocampal regions during intermittent hypoxia. Respir Physiol Neurobiol 136(2-3):91-103, 2003
Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR. Proteomic Identification of 14-3-3zeta as a Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 Substrate: Role in Dimer Formation and Ligand Binding. Mol Cell Biol 23:5376-538, 2003
Thongboonkerd V, Klein JB, Pierce WM Jr., Jevans AW, Arthur JM. Sodium loading changes urinary protein excretion: a proteomic analysis. Am J Physiol Renal Physiol 284(6):F1155-F1163, 2003
Thongboonkerd V, Barati MT, McLeish KR, Benarafa C, Remold-O'Donnell E, Zheng S, Rovin BH, Pierce WM, Epstein PN, Klein JB. Alterations in the renal elastin-elastase system in Type 1 diabetic nephropathy identified by proteomic analysis. J Am Soc Nephrol 15(3):650-62, 2004
Miller KK, Cai J, Ripp SL, Pierce WM Jr, Rushmore TH, Prough RA. Stereo-and regioselectivity account for the diversity of dehydroepiandrosterone (DHEA) metabolites produced by liver microsomal cytochromes p450. Drug Metab Dispos 32(3):305-13, 2004
Thongboonkerd V, Barati MT, McLeish KR, Pierce WM, Epstein PN, Klein JB. Proteomics and diabetic nephropathy. Contrib Nephrol 141:142-54, 2004
Pierce WM, Cai J. Applications of mass spectrometry in proteomics.Contrib Nephrol 141:40-58, 2004
Phone: Coming soon.
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