J. Brad Chaires, PhD
Research Program
Structural Biology
Education Research and Professional Experience 1982 1982-1986 1986-1990 1987 1989-1990 1990-2004 2000-present 2004-present 2004-present 2004-present Selected Awards and Professional Honors 1989-1990 1991-1992 1996 1997-2001 2000 2000, 2003 2000 2004
B.A., University of California at Santa Cruz, Biology, 1972
Ph.D., University of Connecticut, Biophysics, 1978
Postdoctoral Fellow, Yale University, Biophysical Chemistry, 1978-1981
1979-1981
Department of Medicine, Yale University, NIH Postdoctoral Fellow
Full Member of Graduate Faculty, Department of Biochemistry, University of Mississippi Medical Center, Jackson, MS
Assistant Professor, Department of Biochemistry, University of Mississippi Medical Center, Jackson
Associate Professor, Department of Biochemistry, University of Mississippi Medical Center, Jackson
Tenured, Department of Biochemistry, University of Mississippi Medical Center, Jackson
Visiting Scientist, Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry
Professor, Department of Biochemistry, University of Mississippi Medical Center, Jackson
Professor, Joint Appointment, Department of Chemistry, University of Mississippi, Oxford
James Graham Brown Endowed Professor of Biophysics, Division of Hematology-Oncology, University of Louisville, Louisville, KY
Professor with tenure, Department of Medicine, University of Louisville
Senior Scientist, James Graham Brown Cancer Center, University of Louisville
1968
President's Scholar, University of California
Alexander von Humboldt Fellow
Member, National Science Foundation Advisory Panel for the Biophysics Program
Basic Science Teacher Award, University of Mississippi School of Dentistry
Chartered Reviewer, NIH, Molecular and Cellular Biophysics Study Section
Outstanding Chemist, Mississippi Section of the American Chemical Society
Member, National Cancer Institute Program Project Grant Site Visit Team
Member, Committee of Visitors for the Biomolecular Structure and Function and Biomolecular Processes Clusters, Division of Molecular and Cellular Biosciences, National Science Foundation
Chairperson, NIH Bioengineering Research Partnership Review Panel ZRG1 BST-A(50)
Research Interest Physical biochemistry, drug-DNA interactions, high-throughput screening My research interests are in the physical biochemistry of nucleic acids and their interactions. We use spectroscopic and calorimetric methods to measure the thermodynamics of nucleic acid binding interactions and to study the stability of nucleic acid structures. Small molecules that bind selectively to particular nucleic acid structures (triplexes, quadruplexes) are of particular current interest. In addition, we are actively interested in the development of enabling biophysical technologies for the rapid screening of drug-nucleic acid interactions. A novel competition dialysis assay was invented to screen small molecule libraries against an array of defined nucleic acid sequences and structures. This technology will enable us to better define nucleic acids as molecular targets for small molecule therapeutics.
Publications
Qu X, Chaires JB. Analysis of drug-DNA binding data. in: Numerical Computer Methods, Part C, Method in Enzymology, Vol. 321, (L Brand, MJ Johnson, eds.), Academic Press, Inc., pp. 353-69, 2000
Haq I, Jenkins T, Chowdhry BZ, Jinsong Ren, Chaires JB. Parsing the free energy of drug-DNA interactions. In: Energetics of Biological Macromolecules, Part C, Methods in Enzymology, Vol. 323, (MJ Johnson, GK Ackers, eds.), Academic Press, Inc., pp. 373-405, 2000
Ren J, Chaires JB. Preferential binding of 3,3’-diethyloxadicarbocyanine to triplex DNA. J Am Chem Soc (Communication) 122:424-5, 2000
Ren J, Bailly C, Chaires JB. NB-506, an indolcarbazole topoisomerase I inhibitor, binds preferentially to triplex DNA. FEBS Lett 470:355-9, 2000
Ren J, Jenkins TC, Chaires JB. Energetics of intercalation reactions. Biochemistry 39:8439-47, 2000
Qu X, Trent JO, Fokt I, Priebe W, Chaires JB. Allosteric, chiral-selective drug binding to DNA. Proc Natl Acad Sci USA 97:12032-7, 2000
Bailly C, Goossens JF, Laine W, Anizon F, Prudhomme M, Ren J, Chaires JB. Formaldehyde cross-linking of a 2’-aminoglucose Rebeccamycin derivative to both A-T and G-C base pairs in DNA. J Medic Chem 43:4711-20, 2000
Dignam JJ, Qu X, Chaires JB. Equilibrium unfolding of Bombyx mori glycyl-tRNA synthetase. J Biol Chem 276:4028-37, 2001
Chaires JB. Analysis and interpretation of ligand-DNA binding isotherms. In: Drug-Nucleic Acid Interactions, Methods in Enzymology, Vol. 340, (JB Chaires, MJ Waring, eds.), Academic Press, Inc., pp. 3-22, 2001
Ren J, Chaires JB. Rapid screening of structurally selective ligand binding to nucleic acids. Ibid., pp. 99-108
Priebe W, Fokt I, Przewloka T, Chaires JB, Portugal J, Trent JO. Exploiting the anthracycline scaffold for designing DNA targeting agents. Ibid., pp. 529-55
Ren J, Qu X, Dattagupta N, Chaires JB. Molecular recognition of a RNA:DNA hybrid structure. J Am Chem Soc 123:6742-3, 2001
Murr MM, Harting MT, Guelev V, Ren J, Chaires JB, Iverson BL. An octakis-intercalating molecule. Bioorganic Medicinal Chem 9:1141-8, 2001
Alberti P, Ren J, Teulade-Fichou MP, Guittat L, Riou JF, Chaires JB, Helene C, Vigneron JP, Lehn JM, Mergny JL. Interaction of an acridine dimer with DNA quadruplex structures. J Biomolec Struct Dynam 19:505-13, 2001