Andrew N. Lane, PhD

James Graham Brown Endowed Chair of Structural Biology; Professor of Medicine, Chemistry and Biochemistry & Molecular Biology; Leader, Structural Biology Program, James Graham Brown Cancer Center; Director, NMR Facility, James Graham Brown Cancer Center; Associate Director (NMR) Center for Regulatory Environmental Analytical Metabolomics

Research Program
Structural Biology

Education
B.Sc., University College London, England, Biochemistry, 1972-1975
Ph.D., University College London, England, Biochemistry, 1975-1979

Research and Professional Experience

1979-1983
Post-doctoral researcher (with Professor Dr. K. Kirschner), Biophysical Chemistry, Biozentrum, University of Basel, Switzerland

1983-1986
EMBO Fellow (with Professor O. Jardetzky), Stanford Magnetic Resonance Laboratory, Stanford University

1986-1994
MRC Staff Scientist, National Institute for Medical Research, Mill Hill, London, UK

1992-2001
MRC Senior Staff Scientist, National Institute for Medical Research, Mill Hill, London, UK

1994-2001
Senior Staff Scientist, National Institiute for Medical Research, Mill Hill, London, UK

2001-present
Professor of Medicine, University of Louisville, Louisville, KY

Director, NMR Facility, James Graham Brown Cancer Center, University of Louisville

2002-present
Professor of Chemistry (joint), University of Louisville
Professor of Biochemistry & Molecular Biology, University of Louisville

Editorial Board Nucleic Acids Research

Editorial Board Journal of Structural and Functional Genomics

1997-2000
Committee British Biophysical Society

Selected Awards and Professional Honors

Honorary Research Fellow, Department of Biochemistry and Molecular Biology, University College London

James Graham Brown Endowed Chair of Structural Biology, University of Louisville

Program Leader, Structural Biology, James Graham Brown Cancer Center, University of Louisville

Associate Director, CREAM, University of Louisville

Member, NIGMS Metabolomics Network

2009
Mint Jubilee Scientist of the Year - Metabolomics Team

Research Interest

My research is divided into structural and biophysical analyses of macromolecules (DNA and proteins) and their interactions relevant to cancer biology, and stable isotope resolved metabolomics (SIRM) as applied to understanding cancer pathology, and as an approach for target discovery and validation.

Publications
Taylor IA, McIntosh PB, Pala P, Treiber MK, Howell S, Lane AN, Smerdon, SJ. Characterization of the DNA-Binding Domains from the Yeast Cell-Cycle Transcription Factors Mbp1 and Swi4. Biochemistry 39:3943-54, 2000

McIntosh PB, Taylor IA, Frenkiel TA, Smerdon SJ, Lane AN. The influence of DNA-binding on the backbone dynamics of the yeast cell-cycle protein Mbp1. J Biomolec NMR 16:183-96, 2000

Lane AN, Jenkins TC. Thermodynamics of nucleic acids and their interactions with ligands. Q Rev Biophys 33, 255-306, 2000

Lane AN, Hays LM, Tsvetkova N, Feeney RE, Crowe LM, Crowe JH. Comparison of the solution confor-mation and dynamics of antifreeze glycoproteins from Antarctic fish. Biophys J 78:3195-3207, 2000.

Gopal B, Papavinasasundaram KG, Dodson GG, Colston MJ, Major SA, Lane AN. Spectroscopic and thermodynamic characterisation of the transcription antitermination factor NusE and its interaction with NusB from Mycobacterium tuberculosis. Biochemistry 40:920-8, 2001

Alexandrovich A, Czisch M, Frenkiel TA, Moolenaar GF, Goosen N, Sanderson MR, Lane AN. Quaternary structure, thermodynamics and dynamics ofthe C-terminal domain of UvrB. J Biol Ster Dyn 19:219-36, 2001

Lane AN, Kelly G, Ramos A, Frenkiel TA. Determining binding sites in protein-nucleic acid complexes by cross-saturation. J Biomolec NMR 21:127-39, 2001

Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN. NMR structure of the DNA-binding domain of the cell cycle protein, Mbp1 from Saccharomyces cerevisiae. Biochemistry 42:1266-73, 2003

Gyi JI, Gao D, Conn GL, Trent JO, Brown T, Lane AN. The solution structure of a DNA*RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications. Nucleic Acids Res 31:2683-93, 2004

Fan TW, Lane AN, Chekmenev E, Wittebort RJ, Higashi RM. Synthesis and physico-chemical properties of peptides in soil humic substances. J Peptide Res 63(3):253-64, 2004.

Ramos A, Lane AN, Hollingworth D, Fan TW. Secondary structure and stability of the selenocysteine insertion sequences (SECIS) for human thioredoxin reductase and glutathione peroxidase. Nucleic Acids Res 32(5):1746-55, 2004

Fan TW, Lane AN, Higashi RM. An electrophoretic profiling method for thiol-rich phytochelations and metallothioneins. Phytochem Anal 15(3):175-83, 2004

Fan T W-M, Higashi RM, Lane AN. The promise of metabolomics in cancer molecular therapeutics. Current Opin. Molec. Ther 6(6):584-92, 2004 Review

Arumugam S, Gray RD, Lane AN. 1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa. J Biomol NMR 31(3):265-6, 2005

Lane AN, Arumugam S. Improving NMR sensitivity in room temperature and cooled probes with dipolar ions. J Magn Reson 173(2):339-43, 2005

Booth J, Brown T, Vadhia SJ, Lack O, Cummins WJ, Trent JO, Lane AN. Determining the origin of the stabilization of DNA by 5-aminopropynylation of pyrimidines. Biochemistry 44(12):4710-9, 2005

Fan TW, Higashi RM, Lane AN. Integrating metabolomics and transcriptomics for probing SE anticancer mechanisms. Drug Metab Rev 38(4):707-